Journal: International Journal of Stem Cells

Article Title: CRISPR/Cas9 Edited sRAGE-MSCs Protect Neuronal Death in Parkinson’s Disease Model

doi: 10.15283/ijsc18110

Figure Lengend Snippet: Generation and characterization of sRAGE secreting UCB-MSC. (A) The illustration picture represents the gene information of pZDonor-AAVS1 puromycin vector. Each arrow describes certain gene. (B) The illustration of the sRAGE insertion coding sequence. (C) Genome integration was confirmed by Junction PCR with genomic DNAs of UCB-MSCs which were transfected with mock, GFP and sRAGE containing pZDonor-AAVS1 plasmids. (D) Immunoblot analysis of supernatant and extract from UCB-MSC cells transfected with mock (lane 1) and FLAG-tagged sRAGE in pZDonor-AAVS1 vector (lane 2). β -actin loaded as a positive control. The secretion of human sRAGE levels (E) was confirmed with ELISA. ***p<0.001.

Article Snippet: Human soluble receptor for advanced glycosylation end products (sRAGE) ELISA kit (Aviscera Bioscience) was used to quantify the amount of secreted sRAGE.

Techniques: Plasmid Preparation, Sequencing, Transfection, Western Blot, Positive Control, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Advanced glycation end products and their receptors in serum of patients with type 2 diabetes

doi: 10.1038/s41598-021-92630-0

Figure Lengend Snippet: ( A ) Graph of the correlation between the level of glycated hemoglobin HbA1c and the concentration of the SR-AI scavenger receptor in the samples. ( B ) Graph of the correlation between the level of glycated hemoglobin HbA1c and fluorescence of soluble pentosidine. ( C ) Graph of the correlation between the concentration of HDL and AGEs content in samples digested with proteinase K (the competitive ELISA test). Graph of the correlation between the levels of GFR. ( D ) and creatinine. ( E ) and the concentration of the SR-AI receptor in the serum samples. ( F ) Graph of the correlation between the level of uric acid and the fluorescence value of protein-bound total AGEs in the serum samples.

Article Snippet: sRAGE was determined using the ELISA kit for the sRAGE human receptor (MyBioSource, cat. no. MBS766075) according to the manufacturer's instructions.

Techniques: Concentration Assay, Fluorescence, Competitive ELISA

Journal: Scientific Reports

Article Title: Advanced glycation end products and their receptors in serum of patients with type 2 diabetes

doi: 10.1038/s41598-021-92630-0

Figure Lengend Snippet: Graphs of the dependence of AGEs fluorescence or content of sRAGE in the samples and occurrence diabetic complications in patients. The numbers given are the mean value of the results and the standard error of the mean (Mean ± SE). ( A ) The dependence of total AGEs fluorescence on the type of diabetes; (number of subjects = 49; Type 1 (1,167,393 ± 70,632 a.u.), Type 2 (1,517,352 ± 98,785 a.u.)). ( B ) The dependence of total soluble AGEs fluorescence on the occurrence of atherosclerosis; (n = 47; YES (1,581,783 ± 145,154 a.u.), NO (1,179,192 ± 107,389 a.u.)). ( C ) The dependence of total AGEs fluorescence on the occurrence of macroangiopathy; (n = 58; YES (1,511,867 ± 619,228 a.u.), NO (1,105,011 ± 228,314 a.u.)). ( D ) The dependence of total soluble AGEs fluorescence on the occurrence of microangiopathy; (n = 47; YES (1,764,035 ± 207,484 a.u.), NO (1,205,829 ± 68,639 a.u.)). ( E ) The dependence of total soluble AGEs fluorescence on the occurrence of retinopathy; (n = 47; YES (2,749,771 ± 757,703 a.u.), NO (1,327,710 ± 66,108 a.u.)). ( F ) The dependence of soluble pentosidine fluorescence on the occurrence of retinopathy; (n = 47; YES (3,320,508 ± 527,192 a.u.), NO (3,069,839 ± 117,568 a.u.)). ( G ) The dependence of AGEs concentration determined in the competition ELISA test on the occurrence of ischemic stroke; (n = 39; YES (0.9252 ± 0.0687 mg/ml), NO (0.5244 ± 0.0517 mg/ml)). ( H ) The dependence of the concentration of sRAGE receptors on the occurrence of ischemic heart disease; (n = 49; YES (15,467 ± 3967 pg/ml), NO (7404 ± 1628 pg/ml)).

Article Snippet: sRAGE was determined using the ELISA kit for the sRAGE human receptor (MyBioSource, cat. no. MBS766075) according to the manufacturer's instructions.

Techniques: Fluorescence, Concentration Assay, Enzyme-linked Immunosorbent Assay

Journal: Scientific Reports

Article Title: Advanced glycation end products and their receptors in serum of patients with type 2 diabetes

doi: 10.1038/s41598-021-92630-0

Figure Lengend Snippet: The dependence of basic information about the health of patients on the concentration and fluorescence of AGEs and the concentration of AGE receptors. The statistically significant values are shown in red. PL—significance level for Levene’s test; PV – significance level for tke student’s t-test from the analysis of equality of variance; PNV – significance level for the students’s t-test of inequality of variance.

Article Snippet: sRAGE was determined using the ELISA kit for the sRAGE human receptor (MyBioSource, cat. no. MBS766075) according to the manufacturer's instructions.

Techniques: Concentration Assay, Fluorescence, Enzyme-linked Immunosorbent Assay, Indirect ELISA, Competitive ELISA